波利斯·卡洛夫
Boris Karloff
饰：怪人 The Monster (as Karloff)
科林·克利夫
Colin Glenn Clive-Greig
饰：弗兰肯斯坦 Dr. Henry Frankenstein
瓦莱丽·霍布森
Valerie Hobson
饰：弗兰肯斯坦的妻子 Elizabeth Frankenstein
欧内斯特·塞西杰
Ernest Thesiger
饰：怪博士 Dr. Pretorius
爱尔莎·兰切斯特
Elizabeth Lanchester Sullivan
饰：怪人的新娘 Mary Wollstonecraft Shelley,The Monster's Mate
加文·戈登
Gavin Gordon
饰：Lord Byron
道格拉斯·沃尔顿
Douglas Walton
饰：Percy Shelley
尤娜·奥康纳
Una O'Connor
饰：Minnie
E.E. Clive
饰：Burgomaster
卢西恩·普里瓦尔
Lucien Prival
饰：Albert (the butler)
O·P·赫吉
O.P. Heggie
饰：Hermit
德怀特·弗莱伊
Dwight Frye
饰：Karl
Reginald Barlow
饰：Hans
玛丽·戈登
Mary Gordon
饰：Hans' wife
泰德·比林斯
Ted Billings
饰：Ludwig
杰克·柯蒂斯
Jack Curtis
饰：A Hunter
海伦·帕瑞施
Helen Parrish
饰：Communion girl (scenes deleted)
Robert Adair
饰：A hunter (uncredited)
诺曼·安斯利
Norman Ainsley
饰：Little Archbishop (uncredited)
比利·巴蒂
Billy Barty
饰：Little Baby (uncredited)
Frank Benson
饰：Villager (uncredited)
Maurice Black
饰：Gypsy (uncredited)
沃尔特·布伦南
Walter Brennan
饰：Neighbor (uncredited)
Arthur S. Byron
饰：Henry VIII: Little King (uncredited)
约翰·卡拉丁
John Carradine
饰：A hunter (uncredited)
D'Arcy Corrigan
饰：Procession leader (uncredited)
Elspeth Dudgeon
饰：Gypsy's mother (uncredited)
海伦·杰尔姆·埃迪
Helen Jerome Eddy
饰：Gypsy's wife (uncredited)
Neil Fitzgerald
饰：Rudy (uncredited)
Brenda Fowler
饰：A mother (uncredited)
John George
饰：Villager (uncredited)
Helen Gibson
饰：Villager (uncredited)
Marilyn Harris
饰：Girl (uncredited)
Josephine McKim
饰：Little mermaid (uncredited)
Torben Meyer
饰：Man being strangled by the Monster (from flashback during Prolog
Edward Peil Sr.
饰：Villager (uncredited)
玛丽·斯图尔特
Mary Stewart
饰：Neighbor (uncredited)
Lucio Villegas
饰：Priest (uncredited)
Joan Woodbury
饰：Little Queen (uncredited)
Anne Darling
饰：Shepherdess (as Ann Darling)
Grace Cunard
饰：Villager (uncredited)
John Curtis
饰：A hunter (uncredited)
Kansas DeForrest
饰：Little ballerina (uncredited)
Rollo Lloyd
饰：(uncredited)
Sarah Schwartz
饰：Marta (uncredited)
Peter Shaw
饰：Little Devil (uncredited)
Frank Terry
饰：A hunter (uncredited)
Dorothy Vernon
坦普·皮戈特
Tempe Pigott
饰：Auntie Glutz
Edwin Mordant
饰：Coroner
J. Gunnis Davis
A.S. 'Pop' Byron